CHEM 482
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Mechanisms of Molecular Biology
Chemistry and Biochemistry
College of Physical and Mathematical Sciences
Course Description
Second-semester biochemistry. Nucleic acid biochemistry and molecular biology: nucleotide metabolism, chromosome and chromatin structure, DNA structure and replication, RNA transcription and gene expression, protein synthesis and regulation, eukaryotic gene systems, signal transduction.
When Taught
Fall
Min
3
Fixed/Max
3
Fixed
3
Fixed
0
Title
Maintain
Learning Outcome
Maintain faith through challenges and ethical boundaries in reporting and interpreting data.
Title
Design
Learning Outcome
Design a DNA cloning strategy, explain controls necessary, and write procedural plan.
Title
Determine
Learning Outcome
Determine how to perform and interpret an electrophoresis separation of DNA fragments, southern blot, restriction map, polymerase chain reaction, and a DNA sequencing reaction with necessary controls.
Title
Interacts
Learning Outcome
Determine with what a protein interacts: Interpret gel shift assays, Chromatin Immunoprecipitation, DNA footprinting, and yeast two hybrid reporter assays.
Title
Techniques
Learning Outcome
Determine when and how to use techniques that involve RNA: Northern blots, quantitative PCR, microarrays, S1 nuclease and primer extension mapping of the transcriptional start with appropriate controls.
Title
Interpret
Learning Outcome
Determine where a protein is localized in a tissue or cell: interpret a western blot, protein fusion assays.
Title
Mechanism
Learning Outcome
Draw and explain the mechanism of RNA transcription and how RNA is processed, spliced, and regulated. Understand the basic mechanisms of transcriptional regulation using the example of transcription of genes necessary for cholesterol synthesis.
Title
Draw and Explain
Learning Outcome
Draw the structure of DNA, determine the topology of a plasmid, explain the chemical sequence of events that occur during DNA replication and draw structural changes that occur due to mutation.
Title
Sequence
Learning Outcome
Determine the sequence of amino acids in a protein from a series of nucleotides.
Title
Process
Learning Outcome
Draw and explain the process of protein translation and how it is regulated.
